高通量测序技术在水生态污染监测中的应用Application of High-throughput Sequencing Technology in Monitoring of Water Ecological Pollution
景明,陈瑜,高昕,李继影
摘要(Abstract)：
针对张家港某河道水色异常事件,通过高通量测序技术结合显微镜鉴定结果,判断引起河道生态污染的微生物种类,并分析引起该事件的原因。通过Illumina测序,对河道的原核生物及真核生物分别进行测序,真核生物量较多且群落组成较为单一,原核生物量相对较少且群落分布较为均匀,与显微镜检测结果一致。对获得的OTU进行物种注释,得到引起河道生态污染的主要微生物为Pedospumella encystans。本研究为水生态监测提供了新的视角,也证明高通量测序技术在水生态监测中具有较好的应用潜力。
关键词(KeyWords)： 高通量测序;真核生物;原核生物
基金项目(Foundation): 2018年度江苏省环境监测科研基金项目(1810)
作者(Author): 景明,陈瑜,高昕,李继影
DOI: 10.19927/j.cnki.syyt.2021.01.006
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